Nexco | DOPPL - Case Study

Building Confidence in Organoids: How Nexco Analytics Helped DOPPL SA Accelerate Model Selection and Compound Assessment

Multi-dimensional omics analysis delivered faster insights, reliable QC, and actionable results for complex 3D organoid models

"I definitely recommend Nexco Analytics. They are highly skilled, understand our needs, can easily adapt, and are always very fast and efficient."

DOPPL SA, now part of the InSphero Group , engineers industry-standard organoids for disease modeling and personalized medicine. As leaders in advanced 3D cell models, DOPPL provides human-relevant, high-quality, and scalable organoid systems and services for preclinical drug development across diverse areas, including oncology, inflammatory diseases, and ADME-Tox screening. They also develop highly customized organoid lines to model specific tissues or diseases of interest. Omics analysis is crucial for the DOPPL team when characterizing their 3D models and compound screens.

Nexco Analytics supported Aline Roche, Head of R&D at DOPPL, by analyzing multimodal omics data to investigate the signatures of organoids grown from primary tissues across different culture modalities. The aim was to see how well these organoids recapitulated the cell composition and heterogeneity of their tissue of origin. Another aim was to understand the effect on signaling pathway modulation after treatment of organoids with different compounds.

DOPPL lacked in-house omics data analysis expertise.

They struggled with the complexity of the interplay of different parameters like donor variability, culture conditions, and organoid replication

The team needed proper omics quality control by experts in multi-dimensional analysis.

"We lacked in-house expertise to address the complex interplay between different parameters, from donor variability and culture conditions to organoid replication (passaging)"

Nexco Analytics’ analysts first carefully assessed the desired outcomes of the project to ensure optimal analyses were performed and endpoints successfully met.

After high-level discussions about the most appropriate technical analysis approach, the team then performed and dynamically adapted bespoke analyses to provide graphical outputs that successfully answered all of the multidimensional questions posed by the DOPPL team.

"Nexco provided graphical outputs answering all of the multi-dimensional questions we had around our data sets. They offered custom graphical views adapted to specific requests, and provided their input on selecting the best approach to data analysis."

Nexco Analyics delivered rapid, reliable results that meant Aline and the DOPPL team were significantly more confident in their 3D organoid models. It gave them the molecular knowledge to better select models for specific applications and drive their product pipeline forward.

The analysis was essential to validate the reliability of the DOPPL team’s organoid models and allowed them to directly evaluate how closely they resemble patient tissue of origin, without internal omics data analysis expertise.

"The analyses they provided led us to gain stronger knowledge and confidence in our models and helped us better select models for given applications."

Aline Roche, Head of R&D at DOPPL

"The most valuable outcomes of working with Nexco Analytics were the speed of data processing and the reliability of the results and associated QC."

Aline Roche, Head of R&D at DOPPL